Simplified approach for structural elucidation and quantitation for pharmaceutical API and related impurities

Structural elucidation for pharmaceutical impurities involves multitude of analytical techniques, such as NMR, mass spectrometry, infrared spectroscopy etc. A sound decision can be made by compiling the shortlisted candidates from different tools and mapping them appropriately. Nevertheless, highly probable structure(s) can be predicted using HR-MS technique, along with prior background information.

This study has been carried out for structural elucidation of paracetamol and its related impurities followed by quantitation of representative impurities using LCMS-9030 (Shimadzu Corporation, Japan), a Quadrupole Time of flight liquid chromatograph mass spectrometer.

The LCMS-9030 is built using a remarkably stable TOF tube iRefTOFTM, to deliver both high resolution and accurate mass with stability; attributes essential for compound identification, confident formula assignment and quantitation.